Technology breakthrough: visualization of the conversion step from rcDNA towards cccDNA during HBV infection using ANCHOR technology.

Following a long-term collaboration with Dr Michael Kann (Chair of the Department “Fundamental Microbiology and Pathogenicity”, Professor at the University of Bordeaux and Professor at the virology service of the Hospitals of Bordeaux), we have validated ANCHOR tagged Hepatitis B virus (HBV) genomes for imaging and screening applications. Both rcDNA and cccDNA-like specific construct were generated, allowing for the first time the visualization of HBV DNA capsid liberation, and conversion of rcDNA towards cccDNA in living cells. Images showed infection with ANCH-containing pseudo-HBV by triggering the appearance of a fluorescent spot corresponding to the position of the HBV genome. A 20X objective is used, underlining the power of the technology for HCS applications. To use the ANCHOR system: About the Fundamental Microbiology and Pathogenicity department:

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NeoVirTech obtains the exclusive rights to produce and distribute the ANCHOR technology in all domains for research use.

NeoVirTech SAS (NVT) is a biotechnology company dedicated to the design and validation of ANCHOR tagged autofluorescent viruses and viral vectors for imaging and antiviral discovery. Toulouse Tech Transfer SAS (TTT) is a French private technology transfer company developing and commercializing research results by transforming them into innovative products. Following the extension of the license agreement signed in September 2017, NVT acquired the rights to produce, transfer and distribute the ANCHOR technology in all domains for research use only. The ANCHOR technology can automatically detect the position of dynamics of any kind of dsDNA inside living cells. Examples of use include from detection of gene dynamics to plasmid and viral vector localization and accumulation in vivo. For system request: To know more about TTT: toulouse-tech-transfer.comANCHOR3


Live cell imaging of virus infection and replication

People ask me to show dynamics of our autofluorescent viruses in living cells. Here you go. Human epithelial cells were infected with our ANCHOR tagged adenovirus (green dots) and bearing a replication center marker (purple). Stack of images were acquired immediately after infection every 10 min for 48h. This virus has been designed in collaboration with Dr. Harald Wodrich from the University of Bordeaux. Using our ANCHOR system, we can easily visualize the full choreography of adenovirus replication in living cells. We can do the same kind of imaging and quantification with all DNA viruses, oncolytic products, plasmids or gene therapy/vaccine vectors. For more information:


ANCHOR patent granted in the US

Very proud to announce that our technology has been patented in the US. The patent describing the use of the ANCHOR system to visualize and control DNA expression in living cells has been granted under number N°US9611486.

Inserted into the viral DNA, the ANCHOR system triggers its self-detection, each viral DNA forming a fluorescent focus under the microscope (green dots in the picture). Therefore, virus infection and propagation is directly visualized in living cells without fixation, extraction or reagents. For information and request of use:

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NeoVirTech starts a collaboration with Transgene SA

NeoVirTech SAS, a biotechnology company developing autofluorescent viruses to decipher viral infection in vivo and to detect and measure antiviral activities started a collaboration with Transgene SA, a biopharmaceutical company focused on discovering and developing targeted immunotherapies for the treatment of cancer and infectious diseases. Combination of NeoVirTech’s technology with Transgene products provides an unprecedented way to understand and control viral infection directly using microscopy imaging.