First live tracking of HIV-1 based lentiviral vectors in human cell.

We are proud to announce that we obtain great results through a collaborative project with GEG Tech,, a leading provider of lentivirus construct for biotechnology application. NeoVirTech has validated autofluorescent ANCHOR tagging of lentiviral genome (HIV-1 derived) for imaging and screening applications. Both integrating and non-integrating mutants can be visualized thanks to the ANCHOR technology, allowing the first-ever live cell tracking of lentiviral genomes, integrated or not, the observation of vector genome dynamics (see video) and the exact determination of copy number per cell. This new system allows the design of innovative lentiviral vectors and open new ways for gene transfer tool users as well as for R&D in vectorology and virology.

Images show transduced cells with ANCHOR HIV-1 derived lentivirus 24h PT (left) and 48hPT (right). Fluorescent spots correspond to the position of lentivirus genomes. For example, 44 vector genomes copies can be detected in the cell nucleus 24hPT. NeoVirTech thanks Dr. Pierre Cordelier and Dr. Pierre Garcin for expert technical assistance.

To use the ANCHOR system: contact@neovirtech.com. About GEG Tech: https://www.geg-tech.com