Technology breakthrough: visualization of the conversion step from rcDNA towards cccDNA during HBV infection using ANCHOR technology.

Following a long-term collaboration with Dr Michael Kann (Chair of the Department “Fundamental Microbiology and Pathogenicity”, Professor at the University of Bordeaux and Professor at the virology service of the Hospitals of Bordeaux), we have validated ANCHOR tagged Hepatitis B virus (HBV) genomes for imaging and screening applications. Both rcDNA and cccDNA-like specific construct were generated, allowing for the first time the visualization of HBV DNA capsid liberation, and conversion of rcDNA towards cccDNA in living cells. Images showed infection with ANCH-containing pseudo-HBV by triggering the appearance of a fluorescent spot corresponding to the position of the HBV genome. A 20X objective is used, underlining the power of the technology for HCS applications. To use the ANCHOR system: About the Fundamental Microbiology and Pathogenicity department: