Generation of the first autofluorescent Vaccinia virus

Following collaboration with Transgene, a biotechnology company focused on discovering and developing targeted immunotherapies for the treatment of cancers and infectious diseases, we have validated the first poxvirus where the viral DNA is autofluorescent: the ANCHOR Vaccinia Virus (VV DNA in red). Treatments based on Vaccinia Viruses are among Transgene’s lead products. This virus can now be used to understand the behavior of oncolytic vectors and for screening applications in living cells. Microscopy data generated by autofluorescent viruses are a tremendous asset for communication/investor/media. More information:,


Generation of the first autofluorescent animal poxvirus

Following collaboration with Dr Stéphane Bertagnoli from the National Veterinary School of Toulouse, NeoVirTech has validated autofluorescent ANCHOR tagging of an animal poxvirus for imaging and screening applications. Both pathogen and vaccine strains have been generated, allowing live cell tracking of poxvirus genomes dynamics and replication. This achievement will help us discover new antiviral molecules against the poxvirus family, understand the behaviour of vaccine construct or develop and optimize new oncolytic products. Live cell imaging allows us to visualize and measure the dynamics of poxvirus infection and replication.


First live tracking of HIV-1 based lentiviral vectors in human cell.

We are proud to announce that we obtain great results through a collaborative project with GEG Tech,, a leading provider of lentivirus construct for biotechnology application. NeoVirTech has validated autofluorescent ANCHOR tagging of lentiviral genome (HIV-1 derived) for imaging and screening applications. Both integrating and non-integrating mutants can be visualized thanks to the ANCHOR technology, allowing the first-ever live cell tracking of lentiviral genomes, integrated or not, the observation of vector genome dynamics (see video) and the exact determination of copy number per cell. This new system allows the design of innovative lentiviral vectors and open new ways for gene transfer tool users as well as for R&D in vectorology and virology.

Images show transduced cells with ANCHOR HIV-1 derived lentivirus 24h PT (left) and 48hPT (right). Fluorescent spots correspond to the position of lentivirus genomes. For example, 44 vector genomes copies can be detected in the cell nucleus 24hPT. NeoVirTech thanks Dr. Pierre Cordelier and Dr. Pierre Garcin for expert technical assistance.

To use the ANCHOR system: About GEG Tech:


Technology breakthrough: visualization of the conversion step from rcDNA towards cccDNA during HBV infection using ANCHOR technology.

Following a long-term collaboration with Dr Michael Kann (Chair of the Department “Fundamental Microbiology and Pathogenicity”, Professor at the University of Bordeaux and Professor at the virology service of the Hospitals of Bordeaux), we have validated ANCHOR tagged Hepatitis B virus (HBV) genomes for imaging and screening applications. Both rcDNA and cccDNA-like specific construct were generated, allowing for the first time the visualization of HBV DNA capsid liberation, and conversion of rcDNA towards cccDNA in living cells. Images showed infection with ANCH-containing pseudo-HBV by triggering the appearance of a fluorescent spot corresponding to the position of the HBV genome. A 20X objective is used, underlining the power of the technology for HCS applications. To use the ANCHOR system: About the Fundamental Microbiology and Pathogenicity department:

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NeoVirTech obtains the exclusive rights to produce and distribute the ANCHOR technology in all domains for research use.

NeoVirTech SAS (NVT) is a biotechnology company dedicated to the design and validation of ANCHOR tagged autofluorescent viruses and viral vectors for imaging and antiviral discovery. Toulouse Tech Transfer SAS (TTT) is a French private technology transfer company developing and commercializing research results by transforming them into innovative products. Following the extension of the license agreement signed in September 2017, NVT acquired the rights to produce, transfer and distribute the ANCHOR technology in all domains for research use only. The ANCHOR technology can automatically detect the position of dynamics of any kind of dsDNA inside living cells. Examples of use include from detection of gene dynamics to plasmid and viral vector localization and accumulation in vivo. For system request: To know more about TTT: toulouse-tech-transfer.comANCHOR3


Live cell imaging of virus infection and replication

People ask me to show dynamics of our autofluorescent viruses in living cells. Here you go. Human epithelial cells were infected with our ANCHOR tagged adenovirus (green dots) and bearing a replication center marker (purple). Stack of images were acquired immediately after infection every 10 min for 48h. This virus has been designed in collaboration with Dr. Harald Wodrich from the University of Bordeaux. Using our ANCHOR system, we can easily visualize the full choreography of adenovirus replication in living cells. We can do the same kind of imaging and quantification with all DNA viruses, oncolytic products, plasmids or gene therapy/vaccine vectors. For more information: