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Generation of autofluorescent baculoviruses

NeoVirTech announces the generation of autofluorescent baculoviruses and the corresponding patent protection. Following collaboration with Dr Miguel Lopez-Ferber from the IMT Mines Alès, NeoVirTech has validated autofluorescent ANCHOR tagging of an insect virus for imaging and screening applications. Several autofluorescent baculoviruses have been generated allowing live cell tracking of virus genomes dynamics and replication, from single cells to organism. This achievement provides a new rapid and efficient tool to investigate bacculovirus biology, these viruses being used as gene transfer vectors, biopesticide or biomimetic of extremely dangerous pathogens. To know more about what the ANCHOR system can do, contact us at contact@neovirtech.com. To know more about IMT Mines Alès: http://www.mines-ales.fr/

ANCHOR VV

Generation of the first autofluorescent Vaccinia virus

Following collaboration with Transgene, a biotechnology company focused on discovering and developing targeted immunotherapies for the treatment of cancers and infectious diseases, we have validated the first poxvirus where the viral DNA is autofluorescent: the ANCHOR Vaccinia Virus (VV DNA in red). Treatments based on Vaccinia Viruses are among Transgene’s lead products. This virus can now be used to understand the behavior of oncolytic vectors and for screening applications in living cells. Microscopy data generated by autofluorescent viruses are a tremendous asset for communication/investor/media. More information: contact@neovirtech.com, communication@transgene.fr

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Generation of the first autofluorescent animal poxvirus

Following collaboration with Dr Stéphane Bertagnoli from the National Veterinary School of Toulouse, NeoVirTech has validated autofluorescent ANCHOR tagging of an animal poxvirus for imaging and screening applications. Both pathogen and vaccine strains have been generated, allowing live cell tracking of poxvirus genomes dynamics and replication. This achievement will help us discover new antiviral molecules against the poxvirus family, understand the behaviour of vaccine construct or develop and optimize new oncolytic products. Live cell imaging allows us to visualize and measure the dynamics of poxvirus infection and replication.

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First live tracking of HIV-1 based lentiviral vectors in human cell.

We are proud to announce that we obtain great results through a collaborative project with GEG Tech,, a leading provider of lentivirus construct for biotechnology application. NeoVirTech has validated autofluorescent ANCHOR tagging of lentiviral genome (HIV-1 derived) for imaging and screening applications. Both integrating and non-integrating mutants can be visualized thanks to the ANCHOR technology, allowing the first-ever live cell tracking of lentiviral genomes, integrated or not, the observation of vector genome dynamics (see video) and the exact determination of copy number per cell. This new system allows the design of innovative lentiviral vectors and open new ways for gene transfer tool users as well as for R&D in vectorology and virology.

Images show transduced cells with ANCHOR HIV-1 derived lentivirus 24h PT (left) and 48hPT (right). Fluorescent spots correspond to the position of lentivirus genomes. For example, 44 vector genomes copies can be detected in the cell nucleus 24hPT. NeoVirTech thanks Dr. Pierre Cordelier and Dr. Pierre Garcin for expert technical assistance.

To use the ANCHOR system: contact@neovirtech.com. About GEG Tech: https://www.geg-tech.com

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Technology breakthrough: visualization of the conversion step from rcDNA towards cccDNA during HBV infection using ANCHOR technology.

Following a long-term collaboration with Dr Michael Kann (Chair of the Department “Fundamental Microbiology and Pathogenicity”, Professor at the University of Bordeaux and Professor at the virology service of the Hospitals of Bordeaux), we have validated ANCHOR tagged Hepatitis B virus (HBV) genomes for imaging and screening applications. Both rcDNA and cccDNA-like specific construct were generated, allowing for the first time the visualization of HBV DNA capsid liberation, and conversion of rcDNA towards cccDNA in living cells. Images showed infection with ANCH-containing pseudo-HBV by triggering the appearance of a fluorescent spot corresponding to the position of the HBV genome. A 20X objective is used, underlining the power of the technology for HCS applications. To use the ANCHOR system: contact@neovirtech.com. About the Fundamental Microbiology and Pathogenicity department: https://lnkd.in/dn5rSwb